Description
Principle of the Assay: The Mouse HAI-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HAI-1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse HAI-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HAI-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse HAI-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HAI-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms